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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.8
- 수록면
- 483 - 488 (6page)
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This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.
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참고문헌 (14)
참고문헌 신청
[보고서] - World Health Organization / 2012 / Chagas disease (American trypanosomiasis)
[학술지(정기간행물)] - El Sayed NM / 2005 / The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease / Science 309 : 409 ~ 415
[학술지(정기간행물)] - Medina-Acosta E / 1993 / Rapid isolation of DNA from trypanosomatid protozoa using a simple ‘mini-prep’ procedure / Mol Biochem Parasitol 59 : 327 ~ 329
[학술지(정기간행물)] - Towbin H / 1979 / Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications / Proc Natl Acad Sci USA 76 : 4350 ~ 4354
[학술지(정기간행물)] - Porcile PE / 2003 / A refined molecular karyotype for the reference strain of the Trypanosoma cruzi genome project (clone CL Brener) by assignment of chromosome markers / Gene 308 : 53 ~ 65
[학술지(정기간행물)] - El Sayed NM / 2005 / The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease / Science 309 : 409 ~ 415
[학술지(정기간행물)] - Medina-Acosta E / 1993 / Rapid isolation of DNA from trypanosomatid protozoa using a simple ‘mini-prep’ procedure / Mol Biochem Parasitol 59 : 327 ~ 329
[학술지(정기간행물)] - Towbin H / 1979 / Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications / Proc Natl Acad Sci USA 76 : 4350 ~ 4354
[학술지(정기간행물)] - Porcile PE / 2003 / A refined molecular karyotype for the reference strain of the Trypanosoma cruzi genome project (clone CL Brener) by assignment of chromosome markers / Gene 308 : 53 ~ 65
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UCI(KEPA) : I410-ECN-0101-2016-513-001839540