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논문 기본 정보

자료유형
학술저널
저자정보
Samantha Serafin Sevilleno (Sahmyook University) Yoon Ha Ju (Kansas State University) Jung Sun Kim (Rural Development Administration) Franklin Hinosa Mancia (Sahmyook University) Eun Ju Byeon (Rural Development Administration) Raisa Aone Cabahug (Sahmyook University) Yoon‑Jung Hwang (Sahmyook University)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.42 No.3
발행연도
2020.1
수록면
337 - 345 (9page)

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Background C4 plants are efficient in suppressing photorespiration and enhancing carbon gain as compared to C3 plants. Bienertia sinuspersici Akhani is one of the few species in the family Amaranthaceae that can perform C4 photosynthesis within individual chlorenchyma cells, without the conventional Kranz anatomy in its leaf. This plant is salt-tolerant and is well-adapted to thrive in hot and humid climates. To date, there have been no reported cytogenetic analyses yet on this species. Objective This study aims to provide a cytogenetic analysis of B. sinuspersici as the first step in genome sequencing. Methods Fluorescence in situ hybridization (FISH) karyotype analysis was conducted using the metaphase chromosomes of B. sinuspersici probed with 5S and 45S rDNA and Arabidopsis-type telomeric repeats. Results Results of the cytogenetic analysis confirmed that B. sinuspersici has 2n = 2x = 18 consisting of nine pairs of metacentric chromosomes. Two loci of 45S rDNA were found on the distal regions of the short arm of chromosome 7. Nine loci of 5S rDNA were found in the pericentromeric regions of chromosomes 1, 3, 4, 6, and 8, which also colocalized with Arabidopsis-type telomeric repeats; while four loci in the interstitial regions of chromosome 5 and 8 can be observed. The single locus of 5S rDNA that was found in chromosome 8 appears to be hemizygous. Conclusion The FISH karyotype analysis, based on the combination of rDNAs, telomeric tandem repeat markers and C0t DNA chromosome landmarks, allowed efficient chromosome identification and provided useful information in characterizing the genome of B. sinuspersici.

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