지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Electron microscopy is currently the only available technique with a spatial resolution sufficient to identify fine neuronal processes and synaptic structures in densely packed neuropil. For large-scale volume reconstruction of neuronal connectivity, serial block-face scanning electron microscopy allows us to acquire thousands of serial images in an automated fashion and reconstruct neural circuits faster by reducing the alignment task. Here we introduce the whole reconstruction procedure of synaptic network in the rat hippocampal CA1 area and discuss technical issues to be resolved for improving image quality and segmentation. Compared to the serial section transmission electron microscopy, serial block-face scanning electron microscopy produced much reliable three-dimensional data sets and accelerated reconstruction by reducing the need of alignment and distortion adjustment. This approach will generate invaluable information on organizational features of our connectomes as well as diverse neurological disorders caused by synaptic impairments.
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참고문헌 (18)
참고문헌 신청
Blackstad T W / 1965 / Electron microscopy and biological structure research / Tidsskr. Nor. Laegeforen 85:97~103
Briggman K L / 2012 / Volume electron microscopy for neuronal circuit reconstruction / Curr. Opin. Neurobiol 22:154~161
Albert Cardona / 2010 / An Integrated Micro- and Macroarchitectural Analysis of the Drosophila Brain by Computer-Assisted Serial Section Electron Microscopy / PLoS Biology 8(10):e1000502
Denk W / 2004 / Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure / PLoS Biol 2:e329
Frotscher M / 2014 / Fine structure of synapses on dendritic spines / Front. Neuroanat 8:94
Briggman K L / 2012 / Volume electron microscopy for neuronal circuit reconstruction / Curr. Opin. Neurobiol 22:154~161
Albert Cardona / 2010 / An Integrated Micro- and Macroarchitectural Analysis of the Drosophila Brain by Computer-Assisted Serial Section Electron Microscopy / PLoS Biology 8(10):e1000502
Denk W / 2004 / Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure / PLoS Biol 2:e329
Frotscher M / 2014 / Fine structure of synapses on dendritic spines / Front. Neuroanat 8:94
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