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Negative staining electron microscopy facilitates the visualization of small bio-materials such as proteins; thus, many electron microscopists have used this conventional method to visualize the morphologies and structures of biological materials. To achieve sufficient contrast of the materials, a number of imaging parameters must be considered. Here, we examined the effects of one of the fundamental imaging parameters, electron beam exposure time, on electron densities generated using transmission electron microscopy. A single site of a negatively stained biological sample was illuminated with the electron beam for different times (1, 2, or 4 seconds) and sets of micrographs were collected. Computational image processing demonstrated that longer exposure times provide better electron densities at the molecular level. This report describes technical procedures for testing parameters that allow enhanced evaluations of the densities of electron microscopy images.
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참고문헌 (19)
참고문헌 신청
Anderson L J / 1989 / A rapid method for staining large chylomicrons / J. Lipid Res 30 : 1819 ~ 1824
Boekema E J / 2009 / Single particle electron microscopy / Photosynth Res 102 : 189 ~ 196
Brenner S / 1959 / A negative staining method for high resolution electron microscopy of viruses / Biochim. Biophys. Acta 34 : 103 ~ 110
Burgess S A / 2004 / Use of negative stain and single-particle image processing to explore dynamic properties of flexible macromolecules / J. Struct. Biol 147 : 247 ~ 258
De Carlo S / 2011 / Negative staining and cryo-negative staining of macromolecules and viruses for TEM / Micron 42 : 117 ~ 131
Boekema E J / 2009 / Single particle electron microscopy / Photosynth Res 102 : 189 ~ 196
Brenner S / 1959 / A negative staining method for high resolution electron microscopy of viruses / Biochim. Biophys. Acta 34 : 103 ~ 110
Burgess S A / 2004 / Use of negative stain and single-particle image processing to explore dynamic properties of flexible macromolecules / J. Struct. Biol 147 : 247 ~ 258
De Carlo S / 2011 / Negative staining and cryo-negative staining of macromolecules and viruses for TEM / Micron 42 : 117 ~ 131
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